- Pds1 / Esp1 Mutants
- pds1∆
- pds1∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- mad2∆ pds1∆ in nocodazole
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- CLB1 clb2∆ pds1∆
- esp1-ts
- GAL-PDS1-db∆ esp1-ts
- GAL-PDS1-db∆
- PDS1-db∆
- GAL-ESP1 cdc20-ts
CLB1 clb2∆ pds1∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksb2'=0.0003, ksb2"=0.013 (=33% of WT), kspds1"=ks2pds"=0.
Experiments:
Shirayama, M., Toth, A., Galova, M. and Nasmyth, K. (1999). APC(CDC20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5. Nature 402:203-207.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Inviable.
We would expect that, in the absence of Clb2, Cdc5 activation would be delayed even longer, which should not cause problems for pds1∆ cells. We will not be able to account for this mutant until we understand better the roles of Cdc5 and Clb1 in mitotic events.